| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha : |
22/01/2021 |
|
Actualizado : |
14/04/2021 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
|
Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
|
Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
|
DOI : |
10.1016/j.vas.2020.100163 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
|
Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
|
Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
|
Asunto categoría : |
L73 Enfermedades de los animales |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
|
|
Marc : |
LEADER 02681naa a2200373 a 4500
001 1061678
005 2021-04-14
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vas.2020.100163$2DOI
100 1 $aDELPIAZZO, R.
245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy
520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS
653 $aCAMPYLOBACTER FETUS
653 $aMOLECULAR DIAGNOSIS
653 $aMOLECULAR DIAGNOSTICS
653 $aQPCR
700 1 $aBARCELLOS, M.
700 1 $aBARROS, S.
700 1 $aBENTANCOR, L.
700 1 $aFRAGA, M.
700 1 $aGIL, J.
700 1 $aIRAOLA, G.
700 1 $aMORSELLA, C.
700 1 $aPAOLICCHI, F.
700 1 $aPÉREZ, R.
700 1 $aRIET-CORREA, F.
700 1 $aSANGUINETTI, M.
700 1 $aSILVA, A.
700 1 $aSILVEIRA, C.S.
700 1 $aCALLEROS, L.
773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 16 | |
| 1. |  | MACHADO, M.; DE MACÊDO PESSOA, C.R.; PESSOA, A. F. A.; SOUZA MENDES, R. DE; MEDEIROS, R. M. T. DE; DANTAS, A. F. M.; CASTRO, M. B. DE; RIET-CORREA, F. South American rattlesnake (Crotalus durissus spp.) envenomation in dogs in the semiarid region of Brazil. Acta Scientiae Veterinariae, 2020, Volume 48, Article number 521. DOI: https://doi.org/10.22456/1679-9216.104578 Article history: Received: 29 March 2020 Accepted: 17 June 2020 Published: 19 July 2020. Acknowledgments: Thanks are due to Dr. José María Gutiérrez (University of Costa Rica) for the critical reading of the manuscript and suggestions.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
|    |
| 2. | | SOUTO, E.P.F.; PESSOA, C.R.M.; PESSOA, A.F.A.; TROST, M.E.; KOMMERS, G.D.; RIET-CORREA, F.; DANTAS, A.F.M Esophageal pythiosis in an ostrich (Struthio camelus). (Communication). [Ptiose Esofágica em avestrus (Struthio camelus). [Comunicação]. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 1 May 2019, Volume 71, Issue 3, Pages 1081-1084. OPEN ACCESS. Article history:Recebido em 13 de março de 2018/Aceito em 9 de novembro de 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
|  |
| 3. | | DE SOUTO, E.P.F.; MAIA, L.Â.; ASSIS, D.M.; DE MIRANDA NETO, E.G.; KOMMERS, G.D.; DE GALIZA, G.J.N.; RIET-CORREA, F.; DANTAS, A.F.M. Mastite por Pythium insidiosum em éguas. [Mastitis by Pythium insidiosum in Mares.] Acta Scientiae Veterinariae, 2019, Volume 47 (Suppl.1), Article number 387. Doi: 10.22456/1679-9216.91877 Article history: Received: 22 January 2019 / Accepted: 27 April 2019 / Published: 19 May 2019.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
| |
| 4. | | SILVA, T.R.; SOARES, P.C.; DANTAS, A.F.M.; MARQUES, A.V.S.; FILHO, E.F.O.; AGUIAR, G.M.N.; MARQUES, A.L.A.; RIET-CORREA, F. Concentrações sérica e hepática de cobre, ferro, molibdênio e zinco em ovinos e caprinos no estado da Paraíba. [Serum and liver copper, iron, molybdenum and zinc concentration in goats and sheep in the state of Paraíba, Brazil.] Pesquisa Veterinária Brasileira v.38 ,n.7. p. 1313-1316, July 2018. OPEN ACCESS. Article history: Received on July 21, 2017. // Accepted for publication on August 13, 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 5. | | SILVEIRA, A. M.; MOREIRA, E. C.; VAZ, F.; LORENZO, C. DE; DRIEMEIER, D.; FURLAN, F.; DANTAS, A. F. M.; RIET-CORREA, F. Encefalopatia hepática secundária à intoxicação por Tephrosia cinerea em ovinos.(Hepatic encephalopathy secondary to poisoning by Tephrosia cinerea in sheep). Pesquisa Veterinária Brasileira, Rio de Janeiro v. 38, n. 4, p. 635-641, 2018. Article History: Recebido em 28 de abril de 2017.//Aceito para publicação em 17 de maio de 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 6. | | SIMÕES, J.G.; MEDEIROS, R.M.T.; MEDEIROS, M.A.; OLINDA, R.G.; DANTAS, A.F.M.; RIET-CORREA, F. Nitrate and nitrite poisoning in sheep and goats caused by ingestion of Portulaca oleracea. [Intoxicação por nitratos e nitritos em ovinos e caprinos causada pela ingestão de Portulaca oleracea]. Pesquisa Veterinaria Brasileira, 1 August 2018, Volume 38, Issue 8, Pages 1549-1553. OPEN ACCESS Article history: 1 Received on July 27, 2017./Accepted for publication on August 13, 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 7. | | CAMPOS, E.M.; MAIA, L.A.; OLINDA, R.G.; NASCIMENTO, E.M.; MELO, D.B.; DANTAS, A.F.M.; RIET-CORREA, F. Poisoning by Senna obtusifolia in sheep. Pesquisa Veterinaria Brasileira 38(8):1471-1474, agosto 2018. Article History: Received on July 11, 2017.//Accepted for publication on July 26, 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 8. | | SOUTO, E.P. F.; OLINDA, R.G.; ALMEIDA, D.B.B.; ROLIM, V.M.; DRIEMEIER, D,; NOBRE, V.M.T.; RIET-CORREA, F.; ANTÔNIO F. M. DANTAS, A.F.M. Surto de parvovirose cardíaca em filhotes de cães no Brasil. (Outbreak of parvoviral myocarditis in puppies in Brazil). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 94-98, janeiro 2018. Recebido: 30 de Maio de 2016; Aceito: 09 de Dezembro de 2016. Título em inglês: Histopathological and immunohistochemical study of the polyserositis in buffalo (Bubalus bubalis).| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 9. | | SLVEIRA, A. M.; NASCIMENTO, E. M.; KONRADT, G.; NETO, E. G. M.; DRIEMEIER, D.; GALIZA, J. N. G.; DANTAS, A. F. M.; RIET-CORREA, F. Tuberculosis of the central nervous system in cattle in Paraíba, Brazil. [Tuberculose no sistema nervoso central de bovinos na Paraíba.] Pesquisa Veterinaria Brasileira 38(11):2099-2108, 1 November 2018. OPEN ACCESS Article history: Received on June 26, 2018. // Accepted for publication on July 12, 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 10. | | OLINDA, R. G.; MAIA, L. A.; FRADE, M. T. S.; SOARES, M. P.; BARROS, S. S.; DRIEMEIER, D.; RIET-CORREA, F.; DANTAS, A. F. M. Degenerative axonopathy associated with copper deficiency in pigs. (Axonopatia degenerativa associada com deficiência de cobre em suínos). Pesquisa Veterinária Brasileira, Rio de Janeiro v. 37, n. 9, p. 911-915, setembro. 2017. Article history: 1 Received on May 29, 2016 // Accepted for publication on November 11, 2016.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 11. | | SOUTO, E.P.F.; LEITE, A.V.L.; NOBRE, V.M.T.; MIRANDA NETO, E.G.; RIET-CORREA, F.; DANTAS, A.F.M. Morte súbita causada por ruptura de aneurisma em aorta em ruminantes. (Sudden death caused by aortic aneurysm rupture in ruminants). Arquivo brasileiro de medicina veterinária e zootecnia, v.69, n.2, p.398-404, 2017. Article history: Recebido em 10 de setembro de 2016.// Aceito em 30 de setembro de 2016.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 12. | | OLINDA, R,G,; LIMA, J.M.; LUCENA, R.B. DE; VALE, A.M. DO; BATISTA, J.S.; BARROS, C.S.L.DE; RIET-CORREA, F.; DANTAS, A.F.M. Acute aflatoxicosis in swines in Northeastern Brazil .( Aflatoxicose aguda em suínos no Nordeste do Brasil). Acta Scientiae Veterinariae, v. 44(Suppl 1), p. 124-,2016. OPEN ACCESS. History article. Received: 15 October 2015 //Accepted: 17 May 2016// Published: 30 June 2016.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 13. | | OLINDA, R.G..; PENA, H.F.J.; FRADE, M.T.S.; FERREIRA, J.S.; MAIA, L.Â.; GENNARI, S.M.; OLIVEIRA, S.; DANTAS, A.F.M.; RIET-CORREA, F. Acute toxoplasmosis in pigs in Brazil caused by Toxoplasma gondii genotype Chinese 1. Parasitology Research, v. 115,n.7, p. 2561-2566. 2016. Article history: Received: 10 February 2016 /Accepted: 9 March 2016.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 14. | | MAIA, L.A.; OLINDA, R.G.; ARAÚJO, T.F.; FIRMINO, P.R.; NAKAZATO, L.; MIRANDA NETO, E.G.; RIET-CORREA, F.; DANTAS, A.F.M. Cutaneous pythiosis in a donkey (Equus asinus) in Brazil. (Brief Communication) Journal of Veterinary Diagnostic Investigation, 2016, v. 28, no. 4, p. 436?439. Article history: Article first published online: June 6, 2016 / Issue published: July 1, 2016.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 15. | | OLINDA, R. G.; MAIA, L. A.; CARGNELUTTI, J. F.; GOIS, R. C. S.; BATISTA, J. S.; DANTAS, A. F. M.; FLORES, E. F.; RIET-CORREA, F. Swinepox dermatitis in backyard pigs in Northeastern Brazil. Pesquisa Veterinária Brasileira, Brasília, DF v. 36, n. 6, p. 468-472, 2016. Article History:Received on June 11, 2015.//Accepted for publication on March 23, 2016. OPEN ACCESS| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 16. | | OLINDA, R. G.; MEDEIROS, R. M. T.; DANTAS, A. F. M.; LEMOS, R. A. A. DE; RIET-CORREA, F. Intoxicação por Enterolobium contortisdiliquum em bovinos na região Nordeste do Brasil. Pesquisa Veterinária Brasileira, Brasília, DF v. 35, n.1, p. 44-48, jan. 2015. Article history: Recebido em 6 de janeiro de 2015.//Aceito para publicação em 21 de janeiro de 2015.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
| Biblioteca(s): INIA La Estanzuela. |
| |
| Registros recuperados : 16 | |
|
| Expresión de búsqueda válido. Check! |
|
|
